MIROSŁAW KARPIŃSKI, ŁUKASZ ADASZEK, LESZEK DROZD, STANISŁAW WINIARCZYK, PIOTR CZYŻOWSKI
Polimorfizm długości fragmentów restrykcyjnych mitochondrialnego DNA u sarny (Capreolus capreolus)
Restriction fragment length polymorphism of the mitochondrial DNA in roe deer (Capreolus capreolus)

Summary. The aim of this study was to estimate the basic genetic parameters of the studied subpopulation of roe dear (Capreolus capreolus) based on restriction fragment length polymorphism of the mitochondrial DNA. The material for the investigation was collected from two populations of roe deer (Capreolus capreolus) coming from The Forest Inspectorate Bialobrzegi and Zwier-zyniec. To determine the genetic differences between these two populations of roe deer the PCR analysis with the primer pair L14735, H15149 was applied and the comparison of nucleotide sequences of the obtained amplikons of 464 pair in length was made. At the same time, the restric-tion fragments (RFLP) were analysed statistically for three loci using endonucleases HaeIII, HinII, SspI in both studied wild ruminant populations. From the allele frequencies the following were estimated: heterozygosity-H (0.533–0.764), polymorphic information content-PIC (0.375–0.661) and standard distance Ds (0.0094). Using the nucleotide sequences there was developed a phylogenetic tree illustrating genetic diversity within the roe deer populations under investigation. The researches on allele frequencies obtained after the RFLP method proved locus SspI to be the most polymorphic among the three loci selected for genetic variation estimation in roe deer.

Key words: roe deer, mt-DNA, RFLP